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Transcription factors that putatively bind to the sense strand sequence of the BRCA1 promoter in humans were identified, and those showing significantly altered predicted scores for any of the pHaps were selected for further analysis. For each SNP, double-stranded oligonucleotide probes corresponding to the sequences surrounding the polymorphic site were 32 P-radiolabeled and purified using MicroSpin G columns.

Briefly, nuclear proteins from each cell line were quantified with the Bradford protein assay Bio-Rad. For competition experiments, fold molar excess of the unlabeled probe oligonucleotide, the unlabeled corresponding mutant probe or a non-specific DNA probe was added before incubation.

The variants tested and the corresponding antibodies were as follows: rs Brg-1, Oct-1, Nkx Competitions and supershift experiments were carried out by pre-incubating nuclear extracts in binding buffer for 10 min at room temperature, followed by 20 min incubation at room temperature with the radiolabeled double-stranded DNA probes. After electrophoresis, gels were dried and subjected to autoradiographic analysis. The resulting constructs were sequenced to confirm the presence of the expected polymorphic sites, amplified and then purified using QIAfilter Plasmid Kit Qiagen Sciences, Maryland, USA prior to transfection.

Briefly, HeLa cells were co-transfected with ng of pGL3-promoter haplotype-specific constructs encoding a modified firefly luciferase gene and ng of pRL-null vector Promega Corporation encoding the Renilla luciferase gene as an internal standard. The promoterless pGL3-basic vector and pGL3-SV40 control vector, containing the SV40 early promoter, were used as negative and positive controls, respectively. The promoterless pGL3-basic vector was used to measure basal expression levels. Each experiment was performed five times.

Pairs of haplotypes were compared by Student's unpaired t -test. Supplementary Material is available at HMG online. This work was supported by the European Community's Seventh Framework Programme under grant agreement no. We thank Diana Torres and Muhammad U. Rashid for providing DNA samples and supplying data. We thank Antje Seidel-Renkert for expert technical assistance. Easton is the PI of the study. Rogers, Emma McCann.

John Kennedy, David Barton. We wish to thank all the GEMO collaborating groups for their contribution to this study. The content of this manuscript does not necessarily reflect the views or policies of the National Cancer Institute or any of the collaborating centers in the BCFR, nor does mention of trade names, commercial products or organizations imply endorsement by the US Government or the BCFR.

Andersen, and Susanne Kjaergaard for clinical data. Komen Foundation to S. Google Scholar. Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account.

Sign In. Advanced Search. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Abstract. Cox , David G. Oxford Academic. Jacques Simard. Daniel Sinnett. Yosr Hamdi. Penny Soucy. Manon Ouimet. Laure Barjhoux. Carole Verny-Pierre. Lesley McGuffog. Sue Healey.

Csilla Szabo , Csilla Szabo. Mark H. Phuong L. Irene L. Litwin Center for Cancer Genetics. Ontario Cancer Genetics Network. Mads Thomassen. Anne-Marie Gerdes. Maria A. Eitan Friedman. Yael Laitman. Bella Kaufman. Shani S. Per Karlsson. Marie Stenmark Askmalm. Gisela Barbany Bustinza. Katherine L. Susan M. Timothy R. Ute Hamann. Matti A. Ans M. Margreet G. Cora M. Christi J. Peter Devilee. Hans J. Susan Peock. Debra Frost. Gareth Evans. Ros Eeles.

Louise Izatt. Julian Adlard. Joan Paterson. Jacqueline Eason. Andrew K. Marie-Alice Remon. Virginie Moncoutier. Marion Gauthier-Villars. Christine Lasset. Sophie Giraud. Pascaline Berthet. Hagay Sobol. Brigitte Bressac de Paillerets. Olivier Caron. Capucine Delnatte. David Goldgar.

Alex Miron. Hilmi Ozcelik. Saundra Buys. Melissa C. Mary Beth Terry. The Breast Cancer Family Registry. Christian F. Anne-Catharina Dressler.

Muy-Kheng Tea. Thomas V. Oskar Johannsson. Marion Piedmonte. Gustavo C. Jack B. Stephanie Blank. Amanda E. Marco Montagna. Claudine Isaacs. Ignacio Blanco. Simon A. Kirsten B. Rita K. Barbara Wappenschmidt.

Christoph Engel. Alfons Meindl. Nina Ditsch. Norbert Arnold. Dieter Niederacher. Christian Sutter. Dorothea Gadzicki. Britta Fiebig. Trinidad Caldes. Rachel Laframboise. Heli Nevanlinna. Xiaoqing Chen. Jonathan Beesley. Amanda B. Susan L. Yuan C. Fergus J. Xianshu Wang. Paolo Peterlongo. Siranoush Manoukian. Loris Bernard. Paolo Radice. Douglas F. Georgia Chenevix-Trench. Thanks so much for the feature Gutz!! Got more good stuff coming very soon : Just out of interest though, what embed are you using?

Never seen this type anywhere else. Also broken download links for 2 or 3 of the other songs… also, only the first two songs stream for me Firefox Nothing happens? Thanks for alerting me to this.

As I said before, all tracks are working, and downloading, fine on my end. Hopefully we can get this figured out. I couldn't listen to any of the songs at all. It just opens another window with a circle that goes around in circles but nothing opens : Help! I couldn't listen to any of the songs from the last post either. Please do this! TeeTs - Take Control Tonight. Bars Deep - Tomorrow Is Everyday. Doug Bogan - Same Old Minds. Stephen Casciano - Four A. DJ Trademark - Turn On.

Henry Fong - There and Back Pressure.